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Abstract The simple and compact optics of lensless microscopes and the associated computational algorithms allow for large fields of view and the refocusing of the captured images. However, existing lensless techniques cannot accurately reconstruct the typical low-contrast images of optically dense biological tissue. Here we show that lensless imaging of tissue in vivo can be achieved via an optical phase mask designed to create a point spread function consisting of high-contrast contours with a broad spectrum of spatial frequencies. We built a prototype lensless microscope incorporating the ‘contour’ phase mask and used it to image calcium dynamics in the cortex of live mice (over a field of view of about 16 mm 2 ) and in freely moving Hydra vulgaris , as well as microvasculature in the oral mucosa of volunteers. The low cost, small form factor and computational refocusing capability of in vivo lensless microscopy may open it up to clinical uses, especially for imaging difficult-to-reach areas of the body. 

We propose and investigate a minimal mechanism that makes use of differential swelling to modify the critical buckling conditions of elastic bilayer shells, as measured by the knockdown factor. Our shells contain an engineered defect at the north pole and are made of two layers of different crosslinked polymers that exchange free molecular chains. Depending on the size of the defect and the extent of swelling, we can observe either a decreasing or increasing knockdown factor. FEM simulations are performed using a reduced model for the swelling process to aid us in rationalizing the underlying mechanism, providing a qualitative agreement with experiments. We believe that the working principle of our mechanism can be extended to bimetallic shells undergoing variations in temperature and to shells made of pH-responsive gels, where the change in knockdown factor could be changed dynamically.